Specimen containers

Most container types are available from National Procurement Logistics, Canderside Toll, Larkhall.  Items are ordered using the PECOSS on line ordering system.

Blood culture bottles are supplied by Microbiology (call 201 8551 or 956 0439).

The Greiner “Vacuette” system is in use in GGC.  All specimen containers should be robust and leak proof.  The container must be properly closed and not externally contaminated by the contents, as this is hazardous to all staff coming into contact with these samples.

The table below shoes the appropriate specimen container for each specimen/investigation.

Specimen / investigation

Container and comments

Antral washings

Sterile universal container

Aspergillus antigen (galactomannan testing)

Bronchoalveolar lavage/clotted blood (4ml ochre coloured blood tube)

Aspirates and fluids from normally sterile sites (joint, ascites, peritoneal and pleural fluids)

Sterile universal container

Blood cultures

Blood culture Bottles

Paediatric blood culture bottles

Blood for Multiplex antigen detection for pneumococcus, haemophilus and meningococcus

Lavender-topped EDTA tube (4ml adult or 1.8ml paediatric)

Bronchial washings

White-topped sterile container

Bronchoalveolar lavage

White-topped sterile container

Cervical swab

Amies transport medium swab with charcoal

Cerebrospinal fluid (CSF)

White-topped sterile container

Chlamydia + GC PCR

Please contact Regional Virus lab for details 0141 201 8799

Culture for bacterial infections

Pus or a biopsy of the infected tissue is the ideal specimen.  Send in a sterile universal container. If only a small sample of tissue is available, add a few drops of sterile normal saline to prevent drying. Ensure there is NO formalin or other preservative.

If swabs are taken, send Amies transport medium swab with charcoal

Ear swab

Amies transport medium swab with charcoal

Eye swab

Amies transport medium swab with charcoal

For investigation of Chlamydia trachomatis. Please contact Regional Virus lab

Faeces for microscopy, C.diff toxin, C&S and virology

With the spoon provided transfer 10-15ml of faeces, or equivalent volume of fluid, into a Blue-topped sterile universal container

High vaginal swab (HVS)

Amies transport medium swab with charcoal

Intrauterine contraceptive device –IUCD

Send the device in a sterile universal container

Mouth swab

Amies transport medium swab with charcoal

Nasal swab

Amies transport medium swab with charcoal

Pus

Transfer into a sterile universal container.  Only use Amies transport medium swab with charcoal if pus cannot be obtained

MRSA Screening

Liquid eSwab

For Nose & Perineum – duo swab (white top)

For Single site (e.g. nose or throat) – single swab (pink top)

Screening swabs and surface swabs (including, CRO/CPE, neonatal screens)

Amies transport medium swab with charcoal

Seminal fluid for culture

Sterile plain white-top universal container

Serology – e.g. syphilis, ASO, Helicobacter and Legionella Ab

Please contact Regional Virus lab

Skin, nail and hair for mycology

The most suitable method of collecting and transporting specimens of skin, hair and nail is by relevant mycology collection pack. For tissue samples, please indicate on form if fungal infection is suspected.

Amies transport medium swab with charcoal are used for the investigation of Candida infections

Sputum

Sputum from deep expectoration and not saliva is required.  Send specimen in White-topped sterile container (preferably wide neck for sputum)

Throat swab

Amies transport medium swab with charcoal

Tracheal aspirate

White-topped sterile container

Tissues and biopsies

Send in a sterile universal container. If only a small sample of tissue is available, add a few drops of sterile normal saline to prevent drying. Ensure there is NO formalin or other preservative

Tuberculosis

Best specimens are early morning sputum, urine, pus or tissue.  For sputum and urine send 3 early morning specimens taken on consecutive days

Sellotape slide for Enterobious vermicularis

Press Sellotape around the perianal region and transfer to a clean microscope slide.  Place this in a slide box.

Alternatively send 3 dry perianal swabs taken on consecutive day

Urine for routine culture

Clean-voided midstream specimen of urine

Catheter specimen of urine (CSU)

 

DO NOT SEND URINARY CATHETER TIPS

Routine bacteriology:

Primary urine Red top Boric acid containers for all adult specimens

Mini containers available for paediatric samples

Aspirate catheter urines (CSU) form catheter tubing, not from the bag.

Early morning urine for tuberculosis

3 consecutive large Early Morning Urine (EMU) samples

Urethral swab

Amies transport medium swab with charcoal

Vesicles, ulcers and genital lesions

Refer to Regional Virus Laboratory

Water Testing (Legionella)

1L sodium thiosulphate 120mg/L container

Minimum volume required 500ml

Contact GRI Environmental laboratory for collection requirements for other water testing

Wound and ulcer swabs

Amies transport medium swab with charcoal